CBC, Differential with Platelet Count 
Mnemonic CBCD
CPT 85025†
Test Code 2000620
Synonyms CBCD
Test Includes Absolute Basophil Count; Absolute Eosinophil Count; Absolute Lymphocyte Count; Absolute Monocyte Count; Absolute Neutrophil Count
Specimen Whole Blood
Volume 4.0 mL
Container Lavender Top (EDTA) Tube
Special Instructions Invert immediately and mix with anticoagulant 8-10 times.
Specimen Prep Do NOT centrifuge.
Storage Room Temperature, preferred or Refrigerate
Transport Temp Room Temperature
Stability Rm Temp 24 hours
Stability Refrig 24 hours
Stability Frozen Unacceptable
Methodology Automated Cell Counter
Rejection Criteria   
Reference Value Adult Normals (>21y):; WBC: 4.0 - 10.0 x 10e3/µL; Hematocrit: M: 40 - 51%, F: 34 - 45%; Hemoglobin: M: 13.7 – 17.5 g/dL, F: 11.2 – 15.7 g/dL; RBC: M: 4.6 - 6.1 x 10e6/µL, F: 3.9 - 5.2 x 10e6/µL; MCV: 80 - 100 (fl); MCH: M: 25.7 - 32.2 (pg), F: 25.6 - 32.2 (pg); MCHC: 32 - 36 g/dL; RDW: 11.6 - 14.4%; Platelet Count: 163 - 369 x 10e3/µL; MPV: 9.4 - 12.4 (fl); Differential (includes RBC morphology if present); Standard Differential:; Neutrophil: 34 - 71% (1.8 - 7.7 x 10e3/µL); Lymphocyte: 19 - 53% (1.2 - 3.7 x 10e3/µL); Monocyte: 5 - 12% (0.24 - 0.82 x 10e3/µL); Eosinophil: 0 - 7% (0 - 0.54 x 10e3/µL); Basophil: 0 - 1% (0 - 0.08 x 10e3/µL); Immature Granulocyte: <= 1 %; 
Performed Monday - Sunday    
Comments Automated differential performed in all cases except when significant abnormalities detected. Confirmatory or additional tests may be run with additional charges.